What is the purpose of a dual luciferase assay?

The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly.

How does the gene luciferase work?

A commonly used reporter gene is the luciferase gene from the firefly Photinus pyralis. This gene encodes a 61-kDa enzyme that oxidizes D-luciferin in the presence of ATP, oxygen, and Mg(++), yielding a fluorescent product that can be quantified by measuring the released light.

What is luciferase gene?

Luciferases are enzymes that produce light when they oxidize their substrate. The gene for the most common luciferase comes from the firefly, but luciferases from other animals such as the sea pansy Renilla reniformis, the copepod Gaussia princeps, and the ostracod Cypridina noctiluca are also used as reporters.

What is dual reporter?

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample.

Can you eat luciferin?

But, unfortunately, the answer is no. Luciferin is not the way to go about this. First, GoldBio products are not for human or animal consumption. Our products should not be orally ingested or administered as food, medicine or supplements.

Why is it called luciferin?

“Luciferin” got its name from the Latin word “lucifer” (meaning “light-bearing”), which is also a source of the word that is sometimes used as a name of the devil.

Is a substrate for luciferase?

D-Luciferin, Sodium Salt D-Luciferin is a substrate for the enzyme firefly luciferase.

Can humans eat luciferin?

But, unfortunately, the answer is no. Luciferin is not the way to go about this. First, GoldBio products are not for human or animal consumption.

Why is luciferin bad?

Luciferin Toxicity The small size of luciferin also makes it a poor antigen and immune responses to luciferin are unlikely. Luciferin is able to pass the blood brain barrier, the blood placenta barrier and the blood testis barrier, toxicity appears low.