Is titration as accurate as HPLC for determination of vitamin C in supplements?
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Is titration as accurate as HPLC for determination of vitamin C in supplements?
Both HPLC and titration methods accurately analysed vitamin C in SRM 3280, and no statistically significant difference was observed for the results.
Why HPLC is preferred?
The advantages of using HPLC for analysis are that it requires a small sample size, testing can be modified depending on level of quantification needed, and it produces reliable results.
Why we do assay in HPLC?
HPLC methods help analyze drug concentration in biological fluids to evaluate ADME (absorption, distribution, metabolism, and excretion) profile of the drug. The drug discovery phase also involves pharmacology and toxicology assessments of the drug compounds.
What is assay test in HPLC?
Assays based on HPLC are very commonly used to measure the quality of drugs and, according to ICH, certain parameters must be checked to validate the analytical method: linearity, specificity, accuracy, precision (inter and intraday), limit of detection (LOD), limit of quantitation (LOQ) and robustness (ICH-Q2B, 2005; …
What method can be used for vitamin determination?
Table 3
| Pretreatments | Determination Methods | Analytes |
|---|---|---|
| UAE | MEKC | Water- and fat-soluble vitamins |
| LLE | MEKC | Fat-soluble vitamins |
| Dilute and shoot | HPTLC | Vitamins B1, B2, B6 and B12 |
| Extraction, dilute and shoot | PCR, PLS and TLC | Vitamins B1, B6 and B12 |
Which method is used for the extraction of vitamins?
Extraction methods for vitamins from food matrices include acid or alkaline hydrolysis (saponification), enzymatic hydrolysis, direct solvent extraction and solid phase extraction.
What is the difference between purity and assay?
An assay is a quantitative or qualitative measurement of a particular component in a sample. Here, it analyzes the main component of the sample. In contrast, purity is the analysis of impurities in the sample. It is mainly a quantitative measurement.
How many types of assay are there?
Assays can be divided into three main categories based on the type of sample used – ligand-binding assays that measure binding between a ligand and a receptor, immunoassays that detect antibody-antigen binding, and bioassays that measure biological activity in response to certain stimuli.
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