How is lycopene extracted?

Lycopene extract from tomato is produced from a tomato variety with high lycopene content, within the range of 150 to 250 mg/kg. The extract is produced by crushing tomatoes into crude tomato juice that is then separated into serum and pulp. The tomato pulp is then extracted with ethyl acetate.

How lycopene is purified?

The lycopene purification method was developed in two steps. In the first step, carotenoids were extracted from the biomass of B. trispora with petroleum ether/acetone (1:1). In the second step, this partially purified carotenoid extract was further purified by reverse phase chromatography technique.

Which is the best method of analysis of lycopene?

The lycopene content accurately and reproducibly with a correlation coefficient of 0.9996. Results have been compared with HPLC chromatography. The outcome of the study shows that the FTIR method is a good analytical method for quantification of lycopene in tomatoes.

Which solvent mixture is used to isolate lycopene?

Ethyl acetate (PI; 4.4) as an extraction solvent has a dissolving capacity for lycopene and for carotenoids and glycerides too. Anti-solvent extraction can selectively isolate carotenoids and glycerides from a mixture of lycopene, carotenoids, and glycerides.

How is lycopene content measured?

The lycopene concentration is given by: Lycopene (mg/kg fresh wt.) = A503 x 171.7/ W where W is the exact weight of tomato added, in grams.

What is the chemical structure of lycopene?

Lycopene is a water-insoluble, bright red, most common dietary carotenoid synthesized by photosynthetic organisms (plants, algae, and some types of fungus) and chemically characterized by a large unsaturated hydrocarbon chain (tetraterpene) containing 35–40 carbon atoms with chemical formula C40H56 and a melting point …

Why petroleum ether is used in lycopene extraction?

In comparison of hexane extraction with acetone-petroleum ether extraction, acetone-petroleum ether gives higher crude lycopene yield. By results of studies it express that the fruit analyzed contains high concentration of Lycopene. In comparison with other sources, tomato contains maximum amount of lycopene.

Is lycopene water soluble?

It is insoluble in water and nearly insoluble in methanol and ethanol, but is freely soluble in chloroform and tetrahydrofuran. Lycopene is sparingly soluble in ether, hexane, and vegetable oils. A 1\% solution of lycopene in chloroform is clear and has intensive orange-red colour.

How do you test for lycopene?

In order to test the presence of lycopene in tomatoes, tomato puree is dissolved in Petroleum- Ether and ethanol and the solution is allowed to settle. The lycopene-rich solution separates out, resulting in two immiscible liquids.

What’s lycopene good for?

Lycopene is a powerful antioxidant with many health benefits, including sun protection, improved heart health and a lower risk of certain types of cancer. Though it can be found as a supplement, it may be most effective when consumed from lycopene-rich foods like tomatoes and other red or pink fruits.

What is the RF value of lycopene?

The lycopene standards have calculated Rf values of 0.20 each. Lycopene is supposed to carry the color red. It did appear so for most of the parts, with the exception being in some areas it was too light in color. Therefore, the Rf value for the lycopene standard was found to be 0.20.

Is lycopene a terpene?

Lycopene is a carotenoid terpenoid mainly found in tomatoes [145]. Epidemiological data suggests that a regular intake of tomatoes or tomato-derived products may be associated with reduced prostate cancer risk [30].