How does 10X sequencing work?

How does 10X technology work: 10X is a “synthetic long-read” technology and works by capturing an barcoded-oligo coated gel-bead and 0.3x genome copies into a single emulsion droplet (sounds familiar), processing the equivalent of 1 million pipetting steps.

How does 10X single cell sequencing work?

The 10X Genomics Chromium system performs rapid droplet-based encapsulation of single cells using a gel bead in emulsion (GEM) approach. With this method, each gel bead is labeled with oligonucleotides that consist of a unique barcode, a 10 bp UMI, sequencing adapters/primers, and an anchored 30 bp oligo-dT (7).

What is 10X chromium sequencing?

10x Genomics’ Chromium technology partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called GEMs (Gel Bead-In EMulsions). This core technology can be used to partition single cells, nuclei, or high molecular weight gDNA to prepare next generation sequencing libraries in parallel.

What is 10X single cell?

10x Genomics Cloud Analysis enables you to process your single cell gene expression data through a simple web interface, leveraging an optimized, scalable infrastructure for fast results. Users can set up and run Cell Ranger pipelines through Cloud Analysis.

Why is single cell sequencing important?

Single-cell sequencing technologies can detect individual immune cells, thereby distinguishing different groups of immune cells, as well as discovering new immune cell populations and their relationships (Fig. 2). This helps to understand the complex immune system and propose new targets for disease treatment.

What is 10X barcode?

The 10X barcoded gel beads consist of a pool barcodes which are used to separately index each cell’s transcriptome. The individual gel barcodes are delivered to each cell via flow-cytometry, where each cell is fed single-file along a liquid tube and tagged with a 10X gel bead.

Where is 10x Genomics located?

Pleasanton, California
10x Genomics, Inc. is an American company incorporated in 2012 that develops and manufactures integrated systems for whole genome sequencing, exome sequencing and single cell transcriptomics. Its headquarters are located in Pleasanton, California.

Is 10x Genomics a good company?

90\% of employees at 10x Genomics say it is a great place to work compared to 59\% of employees at a typical U.S.-based company. Management is honest and ethical in its business practices.

Is 10X Genomics a good company?

How much does 10X chromium cost?

10X Chromium Controller The average affiliate pricing per sample is $1881.25 for Single Cell 3′ expression, $1826.88 for Single Cell 5′ expression, $1881.25 for Single Cell 5′ Expression + V(D)J immune profiling and $1751.88 for ATAC when performing an 8 sample project.

Where is 10X Genomics located?

Is 10X droplet based?

Currently, there are three prevalent droplet-based systems for high-throughput scRNA-seq, namely, inDrop (Briggs et al., 2018, Klein et al., 2015, Wagner et al., 2018, Zilionis et al., 2017), Drop-seq (Farrell et al., 2018, Macosko et al., 2015), and 10X Genomics Chromium (10X) (Zheng et al., 2017).

https://www.youtube.com/watch?v=hWWkpe4Ewgo