Why polystyrene plate is used in ELISA?

The adsorption to polystyrene microplates was studied with a hapten-conjugated protein (BSA-Ar36) in order to facilitate the analysis of the influence of antibody affinity on desorption during ELISA. Our results show that polystyrene plates adsorb BSA-Ar36 according to the Langmuir isotherm.

What is ELISA plate?

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.

How much protein does it take to coat an ELISA plate?

A concentration range of 1–10 µg/mL of protein, in a volume of 50-100 µL, is a good guide to the level of protein needed to saturate available sites on a polystyrene plate. Commonly used coating solutions are: 50 mM carbonate, pH 9.6; 20 mM Tris-HCl, pH 8.5; and 10 mM PBS, pH 7.2.

Why is ELISA so sensitive?

The sensitivity is the lowest detection level of the marker that the antibody pair used in the ELISA kit can detect. This depends mainly on the affinity of the solid phase antibody according to the law of mass action. Therefore, the use of a high affinity antibody would improve sensitivity.

Does polystyrene bind protein?

At concentrations equal to or higher than their respective critical micelle concentration (CMC) all detergents prevent attachment of proteins to polystyrene. However, coating of proteins on polystyrene plates can be achieved in the presence of detergents at concentrations 10-100 times lower than their CMC values.

What are ELISA plates made of?

ELISAs are typically performed in 96-well or 384-well polystyrene plates, which passively bind antibodies and proteins. It is this binding and immobilization of reagents that makes ELISAs easy to design and perform.

What is the use of ELISA plate?

The plate is synonymous with the enzyme-linked immuno-absorbent assay (ELISA), a standard method of detecting whether a target biochemical substance is present in blood, body fluids or other biological material.

What is Elisa plate made of?

Is ELISA sensitive or specific?

ELISAs are one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng, with sensitivity dependent upon the particular characteristics of the antibody-antigen interaction.

Why is ELISA commonly used?

ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An antibody is a protein produced by the body’s immune system when it detects harmful substances, called antigens.

Is polystyrene a polymer?

Polystyrene (PS) /ˌpɒliˈstaɪriːn/ is a synthetic aromatic hydrocarbon polymer made from the monomer known as styrene. As a thermoplastic polymer, polystyrene is in a solid (glassy) state at room temperature but flows if heated above about 100 °C, its glass transition temperature.