What does DMEM mean?
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What does DMEM mean?
Gibco Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
What is the full form of EMEM and DMEM?
Animal cell culturing is performed to maintain animal cell lines in order to conduct many research activities. There are different types of cell culture media such as Dulbecco’s Modified Eagle Medium (DMEM) and Eagle’s Minimum Essential Medium (EMEM).
What is the composition of DMEM?
11965 – DMEM, high glucose
| Components | Molecular Weight | Concentration (mg/L) |
|---|---|---|
| Amino Acids | ||
| Potassium Chloride (KCl) | 75.0 | 400.0 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 3700.0 |
| Sodium Chloride (NaCl) | 58.0 | 6400.0 |
How much does DMEM cost?
Refine
| Product | Product Name | Price |
|---|---|---|
| SH30022.LS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 1000 mL | 192.19 USD |
| SH30022.01 | DMEM/High glucose with L-glutamine; without sodium pyruvate – 500 mL | 22.25 USD |
| SH30022.FS | DMEM/High glucose with L-glutamine; without sodium pyruvate – 6 x 500 mL | 120.07 USD |
Can you grow HeLa cells in DMEM?
Hela Cells can be grown easily in EMEM as well as in DMEM.
What is the pH of DMEM?
DMEM is buffered for high CO2. Your DMEM probably has 3.7 g sodium bicarbonate which will provide a pH of 7.4 in 10\% CO2. If you remove the high CO2, the sodium bicarbonate takes over and you get a basic pH.
Who invented DMEM?
Minimal Essential Medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture.
Why is DMEM red?
DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. When the media is acidic it turns a yellow-orange color as you describe, and when basic it turns a deep purple (such as if you add bleach).
What is DMEM 10?
Dulbecco’s modified Eagle’s medium (DMEM; GIBCO/Invitrogen) 10\% normal calf serum. 1\% penicillin/streptomycin (Cellgro) 1\% L-glutamine (Cellgro) Filter-sterilize through a 0.22-μm filter.
Why is DMEM high in glucose?
The DMEM original formulation contains 1000 mg/l of glucose, and is used to culture embryonic mouse cells. The use of 4500mg/l of glucose in DMEM High Glucose shows an optimal cell growth for some cell lines.
Why is 5 CO2 used in cell culture?
5\% CO2 for the cell culture is due to the concentration of sodium bicarbonate in the culture medium. 5\%CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.
Who is known as father of tissue culture?
Gottlieb Haberlandt is known as the father of plant tissue culture.