What is the purpose of adding sodium fluoride to the blood sample?

The general conclusion, from the experience of the authors noted above, is that sodium fluoride is an efficient preservative of blood for chemical analysis.

Why is fluoride used as a blood preservative?

Fluoride inhibits enolase, which is far downstream in the glycolytic pathway. Enzymes upstream of enolase remain active and continue to metabolize glucose until substrates are exhausted.

Why is fluoride oxalate used for glucose test?

The number of participants who presented with hypoglycaemia was higher in blood samples stored in plain tubes than in fluoride tubes. This indicates that fluoride-oxalate is a better preservative of blood glucose concentration than plain tube.

What is the mechanism of action of sodium fluoride?

Sodium fluoride kills bacteria that cause caries, such a Streptococcus mutans and lactobacilli 10 by interfering with their metabolic activities that result in the formation of lactic acid. Fluoride ions cause the inhibition of glycolytic and other enzymes involved in bacterial metabolism.

What role does fluoride have in glycolysis?

Fluoride acts primarily by inhibiting enolase in the glycolytic pathway. Fluoride strongly inhibits the enzyme in the presence of inorganic phosphate. The inhibitory species is the fluorophosphate ion, which when bound to magnesium forms a complex with enolase and inactivates the enzyme.

What type of bond is sodium fluoride?

ionic bond
One example of an ionic bond is the formation of sodium fluoride, NaF, from a sodium atom and a fluorine atom. In this reaction, the sodium atom loses its single valence electron to the fluorine atom, which has just enough space to accept it.

How does sodium fluoride inhibit glycolysis?

How does sodium fluoride form?

What is Sodium Fluoride? Sodium fluoride is manufactured by the reaction of hydrofluoric acid with sodium carbonate or sodium hydroxide with the formula NaF. Sodium fluoride solutions are used with hard water insoluble compounds of calcium and magnesium fluoride can form.