How do you estimate the molecular weight of a protein given the number of amino acids?

If the sequence of amino acids is known for any given protein, we can roughly estimate the molecular weight of the protein by multiplying the average molecular weight of amino acids with the total number of amino acids present in the protein. The average molecular weight of an amino acid is known to be 110 Daltons(Da).

How do you calculate protein length?

Whatever program you use to visualize protein structures should have tool to measure the distance between any 2 atoms in the structure. Find the 2 atoms that appear to be the farthest apart, and measure the distance between them. That is the size of the protein in that dimension.

How do you find the molecular weight of a DNA sequence?

The molecular weight or molar mass of any double-stranded DNA fragment can therefore be calculated by multiplying its length (in bp) by 650 and the answer will be expressed as daltons or g/mol.

How do you determine DNA code?

genetic code, the sequence of nucleotides in deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) that determines the amino acid sequence of proteins….genetic code.

DNA triplet	RNA triplet	amino acid
CTT	GAA	glutamic acid
CTC	GAG

How do you determine molecular weight of protein?

An apparent molecular weight (MW) of a protein can be determined from the migration distance of a protein complexed with a strong cationic detergent sodium dodecyl sulfate (SDS) separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

What is the length of a protein?

Protein sizes range from this lower limit to several thousand residues in multi-functional or structural proteins. However, the current estimate for the average protein length is around 300 residues.

What is duration of a protein?

A protein’s lifespan is measured in terms of its half-life and covers a wide range. They can exist for minutes or years with an average lifespan of 1–2 days in mammalian cells. Abnormal or misfolded proteins are degraded more rapidly either due to being targeted for destruction or due to being unstable.

How does a DNA molecule code for a protein?

DNA has the code for a protein which mRNA has to copy and then take that copy out of the nucleus to an other organelle called a ribosome. The ribonucleotides are “read” by translational machinery (the ribosome) in a sequence of nucleotide triplets called codons. Each of those triplets codes for a specific amino acid.

How do you find the molecular size?

Divide both sides by p (rho). (vp= m is equal to v = m / p). The resulting value for v is the volume or three-dimensional size of the molecule.

How do you determine the size of a DNA fragment?

A single DNA fragment (or even a small group of DNA fragments) would not be visible by itself on a gel. By comparing the bands in a sample to the DNA ladder, we can determine their approximate sizes. For instance, the bright band on the gel above is roughly base pairs (bp) in size.

How do DNA fragments move through the agarose gel?

The DNA fragments move through the agarose gel so they are experiencing viscous drag as they move through the gel. This viscous drag is proportional to the mass of the DNA fragment . Let me explain why. The mass of the DNA fragment depends on the length of the DNA fragment.

How do you find the largest and smallest fragments in gel electrophoresis?

The largest fragments are near the top of the gel (negative electrode, where they began), and the smallest fragments are near the bottom (positive electrode). As the gel runs, shorter pieces of DNA will travel through the pores of the gel matrix faster than longer ones.

How many adenine and cytosine bases are present in a DNA segment?

A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is a) 60 (b)120 (c) 240 (d) 480 According to Chargaff rule,