Which enzymes will be produced in a cell in which there is nonsense mutation in the Lac Y gene?

Beta-galactosidaseenzymes will be produced in a cell in which there is a nonsense mutation in the lac y gene . This enzyme is encoded by the gene lacZ. The answer is option (4).

Which enzyme is secreted by lac Z gene?

The lacZ gene encodes β-galactosidase, which degrades lactose. The lacY gene product, lactose permease, transports lactose into the cell, and the lacA gene product, lactose acetylase, has an unknown and not usually necessary function.

Which enzyme is concerned with the entry of lactose in the cell of E coli?

In E. coli, the lacZ gene is the structural gene for β-galactosidase; which is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low.

What do you think would be the effect of a mutation in the lacZ gene?

If this cell had alternative carbon sources to use for ATP production, like glucose or some other sugar, then the major consequence of a mutation in the lac Z gene would be the build up of lactose.

Which enzyme will be produced in the cell?

β -galactosidase is a structural gene present in segment of DNA, which carry Icdes for the synthesis of protein. Mutation in the lac Ygene of E. coli needs residues of cytoplasmic enzyme β -galactosidase. Lactose permease is a membrane protein, which is a major facilitator superfamily.

What enzyme is produced by Z?

The gene Z produces enzyme β-galactosidase for splitting of lactose/galactosidase into glucose and galactose.

What is the function of β galactosidase?

β-Galactosidase has three enzymatic activities (Fig. 1). First, it can cleave the disaccharide lactose to form glucose and galactose, which can then enter glycolysis. Second, the enzyme can catalyze the transgalactosylation of lactose to allolactose, and, third, the allolactose can be cleaved to the monosaccharides.

Which enzyme S will be produced in a cell?

How is lactose transported into the cell?

Lactose permease can be classified as a symporter, which uses the proton gradient towards the cell to transport β-galactosides such as lactose in the same direction into the cell. This form of lactose permease can bind lactose from outside the cell. The enzyme then everts and lactose is transported inward.

How does lactose enter the cell?

Lactose can’t enter the cell unless it’s transported across the membrane by the permease and the permease can only be made if the lac operon is transcribed. Instead, the actual inducer is allolactose, a modified form of lactose that can only be synthesized inside the cell by the enzyme β-galactosidase.

Which enzyme will be produced in a cell?

Is mutation lac operon?

Single mutants of the lac operon Some mutants expressed the lac operon genes constitutively, meaning the operon was expressed whether or not lactose was present in the medium. Such mutant are called constitutive mutants.

https://www.youtube.com/watch?v=hwxx6fRgrLc