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How will you isolate hydrocarbon-degrading bacteria?

How will you isolate hydrocarbon-degrading bacteria?

The hydrocarbon degraders which are stored on glycerol stock were subjected to its efficiency of crude oil degradation. The isolates were single streaked on BHA plates overlaid with 100 µl of crude oil and on plain NA plates and were incubated at 25°C for 14 days and 37°C for 24 – 48 hrs respectively.

What is hydrocarbon-degrading bacteria?

Petroleum hydrocarbon-degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Bacteria displaying such capabilities are often exploited for the bioremediation of petroleum oil-contaminated environments.

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How do you make a Bushnell Haas medium?

Preparation Instructions Suspend 23.27 grams of Bushnell Haas Agar in 1000 mls of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.

How do bacteria break down hydrocarbons?

Just like your automobile, these marine-dwelling bacteria and fungi use the hydrocarbons as fuel—and emit the greenhouse gas carbon dioxide (CO2) as a result. In essence, the microbes break down the ring structures of the hydrocarbons in seaborne oil using enzymes and oxygen in the seawater.

Which bacteria are generally used to degrade derivatives of oils?

Recent studies have identified bacteria from more than 79 genera that are capable of degrading petroleum hydrocarbons (Tremblay et al., 2017); several of these bacteria such as Achromobacter, Acinetobacter, Alkanindiges, Alteromonas, Arthrobacter, Burkholderia, Dietzia, Enterobacter, Kocuria, Marinobacter.

Which bacteria is used for bioremediation?

Deinococcus radiodurans is a radiation-resistant extremophile bacterium that is genetically engineered for the bioremediation of solvents and heavy metals.

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What enzymes break down hydrocarbons?

The bacterium Pseudomonas putida oxidizes various alkanes primarily with a membrane-bound enzyme called AlkB that forms a hydrophobic pocket attractive to hydrocarbon-based substrates. The enzyme converts oxygen to free radicals to break down the trapped substrates.

How do fungi degrade hydrocarbons?

Hydrocarbons, as the most organic pollutants, are degraded by fungi mainly under aerobic conditions; in fact, fungi act through enzymes which oxidize hydrocarbons to form water and non-toxic or less toxic residues.

How do you make mineral salt agar?

Media and culture condition: Mineral salts medium (MSM) (Zajic and Supplisson, 1972) was prepared by dissolving 1.8 g K2HPO4, 4.0 g NH4 CI, 0.2 g MgSO4. 7H2O, 0.1 g NaCl, 0.01 g FeSO4. 7H2O in 1 L of distilled water. Bacteriological agar was added (15 g L-1) to the solution where solid basal medium is required.

How is oil contamination being degraded by microbes?

There are the two main approaches to oil spill bioremediation: (a) bioaugmentation, in which known oil-degrading bacteria are added to supplement the existing microbial population, and (b) biostimulation, in which the growth of indigenous oil degraders is stimulated by the addition of nutrients or other growth-limiting …

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What process do microorganisms use to break down pollutants?

Bioremediation uses micro-organisms to reduce pollution through the biological degradation of pollutants into non-toxic substances. This can involve either aerobic or anaerobic micro-organisms that often use this breakdown as an energy source.